|
KMID : 0363320060270040864
|
|
Journal of Korean Oriental Internal Medicine
2006 Volume.27 No. 4 p.864 ~ p.878
|
|
|
Regulatory Effect of Inflammatory Cytokines Secretion and Hypoxia-inducible Factor-1{alpha} Activation by Panax ginseng
|
|
Cho Cheol-Won
Lee Seung-Hee
Kim Dong-Woung
Lee Seong-Kyun
Song Bong-Keun
|
|
|
Abstract
|
|
|
Purpose : Panax ginseng(PG) is considered to have salutary effects and stimulant actions on physical capacity. However, the effects of PG on the inflammatory cytokine secretion and hypoxia condition are still not understood. This study wasto elucidate the effect of PG on inflammatory cytokine secretion such as interleukin (IL)-1, IL-6, granulocyte macrophage colony stimulating factor (GM-CSF), and tumor necrosis factor (TNF)-{alpha}. Also, the effects on the expression of vascular endothelial growth factor (VEGF) and hypoxia-inducible factor 1 (HIF-1) were measured.
Methods : The water extract of PG was administrated to HMC-1 cells before phorbol myristate acetate (PMA)+A23187 treatment. IL-1{beta}, IL-6, TNF-{alpha}, GM-CSF, and VEGF secretion were measured by a modified enzyme-linked immunosorbent assay (ELISA). HIF-1 activation was measured by transcription factor enzyme-linked immunoassay (TF-EIA).
Results : PG significantly decreased secretion of IL-1{beta}, IL-6, TNF-{alpha}, and GM-CSF in PMA+A23187-induced HMC-1 cells. VEGF secretion was not changed but HIF-1 activation was decreased by the treatment of PG.
Conclusions : PG inhibited the secretion of inflammatory cytokines, which impliesPG might contribute to treatment of mast cell-mediated inflammatory disease. Also, PG inhibited PMA+A23187-induced HIF -1{alpha}activation} and DNA-binding activity for HIF-1. Therefore, these data demonstrate that PG modulates inflammatory cytokines through inhibition of HIF-1{alpha}activation} activation.
|
|
|
KEYWORD
|
|
|
Panax ginseng, inflammatory cytokines secretion, hypoxia-inducible factor-1 (HIF-l){alpha}, anti-inflammatory
|
|
|
FullTexts / Linksout information
|
|
|
|
|
Listed journal information
|
|
|
|